Grazing incidence x-ray diffraction studies of lipid-peptide mixed monolayers during shear flow

Bera, Pradip Kumar and Kandar, Ajoy Kumar and Krishnaswamy, Rema and Imp´eror-Clerc, Marianne and Pansu, Brigitte and Constantin, Doru and Maiti, Santanu and Sanyal, Milan K. and Sood, A. K. (2019) Grazing incidence x-ray diffraction studies of lipid-peptide mixed monolayers during shear flow. Cornell University. pp. 1-20. (Submitted)

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Abstract

Grazing Incidence X-ray Diffraction (GIXD) studies of monolayers of biomolecules at the air-water interface give quantitative information of in-plane packing, coherence lengths of the ordered (diffracting) domains and orientation of hydrocarbon chains of the model mem branes. Rheo-GIXD measurements revel quantitative changes in the monolayer under shear. Here we report GIXD studies of monolayers of Alamethicin peptide, DPPC lipid and their mixtures at the air-water interface under the application of steady shear stresses. The Alame thicin monolayer and the mixed monolayer show flow jamming transition. On the other hand, pure DPPC monolayer under the constant stress flows steadily with a notable enhancement of 1 arXiv:1908.00228v1 [cond-mat.soft] 1 Aug 2019 area/molecule, coherence length, and the tilt angle with increasing stress, suggesting fusion of domains during flow. The DPPC-Alamethicin mixed monolayer shows no significant change in the area/DPPC molecule or in the DPPC chain tilt angle but the coherence length of both phases (DPPC and Alamethicin) increases suggesting that the domains of individual phases aremergingtobiggersizepromotingmoreseparationofphasesinthesystemduringflow. Our results show that Rheo-GIXD has the potential to explore in-situ molecular structural changes under rheological conditions for a diverse range of confined biomolecules at the interfaces.

Item Type:	Article
Authors:	Bera, Pradip Kumar and Kandar, Ajoy Kumar and Krishnaswamy, Rema and Imp´eror-Clerc, Marianne and Pansu, Brigitte and Constantin, Doru and Maiti, Santanu and Sanyal, Milan K. and Sood, A. K.
Uncontrolled Keywords:	X-ray, Grazing Incidence X-ray Diffraction (GIXD), Alamethicin, DPPC, DPPC-Alamethicin,
Subjects:	Natural Sciences > Physics
Divisions:	Azim Premji University - Bengaluru > School of Arts and Sciences
Full Text Status:	Public
Related URLs:	Google Scholar
URI:	http://publications.azimpremjiuniversity.edu.in/id/eprint/2049
Publisher URL:	https://arxiv.org/abs/1908.00228

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