Production and characterization of bioemulsifier by Parapedobacter indicus

Devale, Anushka and Sawant, Rupali and Pardesi, Karishma and Perveen, Kahkashan and Khanam, Mehrun NIsha and Shouche, Yogesh S. and Mujumdar, Shilpa (2023) Production and characterization of bioemulsifier by Parapedobacter indicus. Frontiers in Microbiology, 14. ISSN 1664-302X

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Abstract

The current study evaluated Parapedobacter indicus MCC 2546 for its potential to produce a bioemulsifier (BE). Screening methods performed for BE production by P. indicus MCC 2546 showed good lipase activity, positive drop collapse test, and oil-spreading activity. Furthermore, it showed maximum emulsification activity (225 EU/ml) and emulsification index (E 24 50%) at 37°C in Luria Bertani broth at 72 h with olive oil as a substrate. The optimal pH and NaCl concentration for maximum emulsification activity were 7 and 1%, respectively. P. indicus MCC 2546 lowered the surface tension of the culture medium from 59.65 to 50.42 ± 0.78 mN/m. BE produced was composed of 70% protein and 30% carbohydrate, which showed the protein–polysaccharide nature of the BE. Furthermore, Fourier transform infrared spectroscopy analysis confirmed the same. P. indicus MCC 2546 showed a catecholate type of siderophore production. This is the first report on BE and siderophore production by the genus Parapedobacter.

Item Type:	Article
Authors:	Devale, Anushka and Sawant, Rupali and Pardesi, Karishma and Perveen, Kahkashan and Khanam, Mehrun NIsha and Shouche, Yogesh S. and Mujumdar, Shilpa
Document Language:	Language English
Uncontrolled Keywords:	bioemulsifier, Parapedobacter, heavy metal resistance, bioremediation, BS/BE
Subjects:	Natural Sciences > Chemistry & allied sciences Natural Sciences > Life sciences; biology > Biochemistry Natural Sciences > Life sciences; biology > Microorganisms, fungi & algae
Divisions:	Azim Premji University - Bengaluru > School of Arts and Sciences
Full Text Status:	Public
URI:	http://publications.azimpremjiuniversity.edu.in/id/eprint/7039
Publisher URL:	https://doi.org/10.3389/fmicb.2023.1111135

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